ABSTRACT
Wound swab culture is the most frequently employed method of confirming wound infection. A regular bacteriological review of infected wounds is necessary to provide qualitative health care particularly when blind treatment is a necessity as in underdeveloped and developing nations. Materials and Methods: A total of 614 Wound swabs sample were received in the department during the study period. Direct Gram staining of the specimens were done after which they were inoculated in Blood agar and MacConkey agar plates and antibiotic sensitivity was done according to CLSI guideline. Result: A total of 496 strains were isolated out of which 232 (46.77%) were Gramnegative bacilli and 264(53.23%) were Gram-positive cocci. Out of the 466 culture positive samples, 29 samples showed polymicrobial growth. E coli was the most common pathogen isolated. Of the 156 isolates of Staphylococcus aureus 68 was from ward and 88 from Out Patient Department (OPD) of which 31(45.58%) and 30(34.09%) were determined to be methicillin resistant (MRSA) respectively. Out of 95 isolates of Coagulase Negative Staphylococcus(CoNS ), 56 was from ward and 39 from OPD. Methicillin Resistant Staphylococcus (MRCoNS) prevalence rate was 46 (82.14%) and 28(71.79%) for ward and OPD respectively. The gram negative isolates were most sensitive to imipenem and it was least sensitive to cephalosporin groups of antibiotics. Conclusion: The most commonly isolated pathogen from wound swab specimens was Gram positive bacteria but 46.77% of the isolates were Gram negative bacteria so antimicrobial coverage for Gram negative bacteria should be included in treatment of wound infection.